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4 KwaZulu-Natal Research Institute for TB and HIV (K-RITH), Durban, South Durban, South Africa; National Health Laboratory Service, Durban, South Africa. In this study, we used whole genome sequencing and dating.
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Background: The continued advance of antibiotic resistance threatens the treatment and control of many infectious diseases. Understanding the origins of drug resistance in this fatal outbreak of XDR will inform the control and prevention of drug-resistant TB in other settings. In this study, we used whole genome sequencing and dating analysis to determine if XDR-TB had emerged recently or had ancient antecedents. Methods and findings: We performed whole genome sequencing and drug susceptibility testing on clinical isolates of Mycobacterium tuberculosis collected in KwaZulu-Natal from to , in addition to three historical isolates, collected from patients in the same province and including an isolate from the Tugela Ferry XDR outbreak, a multidrug-resistant MDR isolate from , and a pansusceptible isolate from We utilized an array of whole genome comparative techniques to assess the relatedness among strains, to establish the order of acquisition of drug resistance mutations, including the timing of acquisitions leading to XDR-TB in the LAM4 spoligotype, and to calculate the number of independent evolutionary emergences of MDR and XDR.
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Durban is the third most populous city in South Africa after Johannesburg and Cape Town and In a ship from the Dutch East India Company named ‘Stavenisse’ wrecked off the eastern coast of South Africa. The modern city of Durban thus dates from when the settlement was established on the northern.
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Durban forms part of the eThekwini Metropolitan Municipality , which includes neighboring towns and has a population of about 3. Archaeological evidence from the Drakensberg mountains suggests that the Durban area has been inhabited by communities of hunter-gatherers since , BC. These people lived throughout the area of present-day KwaZulu-Natal until the expansion of Bantu farmers and pastoralists from the north saw their gradual displacement, incorporation or extermination.
Little is known of the history of the first residents, as there is no written history of the area until it was sighted by Portuguese explorer Vasco da Gama , who sailed parallel to the KwaZulu-Natal coast at Christmastide in while searching for a route from Europe to India. He named the area “Natal”, or Christmas in Portuguese. Some of the survivors made their way to the Bay of Natal Durban where they were taken in by Abambo tribe under leadership of Chief Langalibale.
The crew became fluent in the language of the tribe and witnessed their customs. They told that the land where the Abambo people lived was called Embo by the natives and that the people were very hospitable. On the 28th of October the galiot ‘Noord’ traveled from Table Bay to the Bay of Natal for the purpose to fetch the survivors of the crew and to negotiate a deal to purchase the bay. A formal contract was drawn up by Laurens van Swaanswyk and signed by the Chief of the Abambo people, the crew of the Stavenisse acted as translators.
On a return trip to the Cape in , they were caught in a very bad storm and decided to risk the Bar and anchor in the Bay of Natal. The crossing went off well and they found safe anchor from the storm.